Simple, quantitative primer-extension PCR assay for direct monitoring of microRNAs and short-interfering RNAs
نویسندگان
چکیده
منابع مشابه
Simple, quantitative primer-extension PCR assay for direct monitoring of microRNAs and short-interfering RNAs.
There has been a surge of interest in the biology of microRNAs and the technology of RNA interference. We describe a simple, robust, inexpensive assay for quantitative analysis of microRNAs and short-interfering RNAs. The method relies on primer extension conversion of RNA to cDNA by reverse transcription followed by quantitative, real-time PCR. Technical parameters critical to the success of t...
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ژورنال
عنوان ژورنال: RNA
سال: 2005
ISSN: 1355-8382
DOI: 10.1261/rna.2148705